Measurement

Part:BBa_J119139:Design

Designed by: Claire Shinneman   Group: Eckdahl Lab   (2013-01-22)


Device for Testing New Promoters via Golden Gate Assembly


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 750
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 750
    Illegal NotI site found at 756
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 750
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 750
    Illegal XbaI site found at 765
    Illegal AgeI site found at 796
    Illegal AgeI site found at 904
    Illegal AgeI site found at 2233
    Illegal AgeI site found at 2345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 72
    Illegal BsaI site found at 1583
    Illegal BsaI.rc site found at 777


Design Notes

None.


Source

BsmBI GGA of J119136 with PCR product from J100103.

References